Kia ora Stephan,

Thank you for your follow up questions to OIA24-0905 received on 21 February 2025.

Your request below will be considered, and a decision provided in accordance with the requirements of the Official Information Act 1982.

If you have any questions regarding this request, please email [email address]

Ngâ mihi,

Official Information Act Team
Government Services | Public Affairs
Ministry for Primary Industries – Manatû Ahu Matua
Charles Fergusson Building, 38-42 Bowen Street | PO Box 2526 | Wellington 6140 | New Zealand
Web: www.mpi.govt.nz

-----Original Message-----
From: Stephan Hokke <[FOI #30166 email]>
Sent: Friday, 21 February 2025 4:28 am
To: Official Information Act <[email address]>
Subject: Official Information request - PCR count plateaus at about 32. Do the MPI machines plateau the same?

[You don't often get email from [FOI #30166 email]. Learn why this is important at https://aka.ms/LearnAboutSenderIdentific... ]

Dear Fleur Francois,
Director, Diagnostics, Readiness and Surveillance

I made a request earlier "Protocol and results for tests done to detect chicken flu recently at Otago chicken farm" in which you reported that the cycle count was 45. Yet the plateau is at about 32. So why go to 45?

My reference is:https://assets.publishing.service.gov.uk...

Figure 1 on page 5 shows the plateau.

Therefore. In the recent testing for the Otago chicken farms, what would be the result at 32 cycles?

Also, does your PCR equipment plateau the same as per figure 1 on page 5? Are you able to run such a calibration test?

My understanding is that science does require the equipment be calibrated and thus ensure results are repeatable on different equipment.

Yours faithfully,

Stephan Hokke

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