Protocol and results for tests done to detect chicken flu recently at Otago chicken farm.
Stephan Hokke made this Official Information request to Ministry for Primary Industries
The request was partially successful.
From: Stephan Hokke
Dear Ministry for Primary Industries,
I was shocked that there was a positive result in your tests on the chicken farm in Otago. I wish to be sure that your tests are robust and valid and not a random outlier.
Therefore:
1) What report sparked the sending of your team to the chicken farm (copy please)
2) Were the people who took the sample trained in the procedure of getting a sample? Give their name as an identifier such as MPI sampler #001, their qualifications, the chain of custody of the sample from the chicken farm to the testing equipment.
3) How many samples?
4) What method was used to detect the flu? List the machines used, operater's names as an identifier such as MPI tester #001 and their qualifications and experience.
5) How many cycles were used on the sample?
6) How did you match the result to the standard? Please supply the report that the tester provided to you.
7) How can you prove that that standard causes that flu?
8) Please supply all email threads (with names "search and replaced" by an identifier such as emailer#001 etc)
I am sure you have a great team working on this and thus I expect you will supply all the needed confirmation with a minimum of delay that will confirm my confidence that this matter is in good hands.
Yours faithfully,
Stephan Hokke
.
From: Official Information Act
Ministry for Primary Industries
Kia ora Stephan,
Thank you for your official information request received on 4 December 2024.
Your request below will be considered, and a decision provided in accordance with the requirements of the Official Information Act 1982.
Please note that, in accordance with section 2(1) of the OIA, the period from 25 December to 15 January is excluded from the definition of “working day”.
If you have any questions regarding this request, please email [email address]
Ngâ mihi,
Official Information Act Team
Government Services | Public Affairs
Ministry for Primary Industries – Manatû Ahu Matua
Charles Fergusson Building, 38-42 Bowen Street | PO Box 2526 | Wellington 6140 | New Zealand
Web: www.mpi.govt.nz
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From: Official Information Act
Ministry for Primary Industries
Kia ora Stephan,
On behalf of Michelle Morehu, Director Government Services, please find
attached an update regarding your Official Information Act request.
Ngā mihi
Official Information Act Team
Government Services | Public Affairs
Ministry for Primary Industries | Charles Fergusson Building, 34-38 Bowen
Street | PO Box 2526 | Wellington | New Zealand
Telephone: 0800 00 83 33 | Email: [1][email address] |
Web: [2]www.mpi.govt.nz
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References
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1. mailto:[email address]
2. http://scanmail.trustwave.com/?c=8934&am...
Stephan Hokke left an annotation ()
MPI respond saying they need more time because of the large amount of information required. They set a new date of 24 February, if not earlier, to comply with my request.
From: Official Information Act
Ministry for Primary Industries
Tēnā koe Stephan,
On behalf of Fleur Francois, Director, Diagnostics, Readiness and
Surveillance, please find attached a response to your Official Information
Act request.
Ngā mihi,
Official Information Act Team
Government Services | Public Affairs
Ministry for Primary Industries | Charles Fergusson Building, 34-38 Bowen
Street | PO Box 2526 | Wellington | New Zealand
Telephone: 0800 00 83 33 | Email: [1][email address] |
Web: [2]www.mpi.govt.nz
show quoted sections
References
Visible links
1. mailto:[email address]
2. http://www.mpi.govt.nz/
Stephan Hokke left an annotation ()
The cycles was 45 when it should be 40:
https://www.vet.cornell.edu/animal-healt...
I recall that the inventor of PCR (Kary Mullis) indicated 32 cycles was a max which is validated by the UK govt:
https://assets.publishing.service.gov.uk...
The process plateaus at about 32 so why go higher?
Google "maximum cycles for BioRad CFX96 Real-Time PCR Detection System" yeilds
"What is the optimum number of cycles for PCR?
PCR steps of denaturation, annealing, and extension are repeated (or “cycled”) many times to amplify the target DNA. The number of cycles is usually carried out 25–35 times but may vary upon the amount of DNA input and the desired yield of PCR product."
Again: Why 45 cycles?
As to the proof that the standard against which the PCR result is tested to prove it is a flu, they seemed to assume the standard is the cause of the Flu, but did not prove it. This is getting technical so it would be best for a technician to look at this data and see if it makes sense.
From: Stephan Hokke
Dear Fleur Francois,
Director, Diagnostics, Readiness and Surveillance
Thank you for your thorough response.
The Ct count of 45 caused me to take pause. Perhaps your machines plateau at a higher count as per the UK govt the plateau is at about 32 so why go above that level? I am sending a new request to ascertain that point.
I am not yet convinced that the standard you used is the actual cause of the Avian Flu. Is there a paper that proves this by use of Koch's Postulate?
Yours sincerely,
Stephan Hokke
Things to do with this request
- Add an annotation (to help the requester or others)
- Download a zip file of all correspondence
Stephan Hokke left an annotation ()
MPI received the request and notified me the holiday weeks do not count in the turnaround time for the request.
Link to this