PCR count plateaus at about 32. Do the MPI machines plateau the same?
Stephan Hokke made this Official Information request to Ministry for Primary Industries
The request was partially successful.
From: Stephan Hokke
Dear Fleur Francois,
Director, Diagnostics, Readiness and Surveillance
I made a request earlier "Protocol and results for tests done to detect chicken flu recently at Otago chicken farm" in which you reported that the cycle count was 45. Yet the plateau is at about 32. So why go to 45?
My reference is:https://assets.publishing.service.gov.uk...
Figure 1 on page 5 shows the plateau.
Therefore. In the recent testing for the Otago chicken farms, what would be the result at 32 cycles?
Also, does your PCR equipment plateau the same as per figure 1 on page 5? Are you able to run such a calibration test?
My understanding is that science does require the equipment be calibrated and thus ensure results are repeatable on different equipment.
Yours faithfully,
Stephan Hokke
From: Official Information Act
Ministry for Primary Industries
Kia ora Stephan,
Thank you for your follow up questions to OIA24-0905 received on 21 February 2025.
Your request below will be considered, and a decision provided in accordance with the requirements of the Official Information Act 1982.
If you have any questions regarding this request, please email [email address]
Ngâ mihi,
Official Information Act Team
Government Services | Public Affairs
Ministry for Primary Industries – Manatû Ahu Matua
Charles Fergusson Building, 38-42 Bowen Street | PO Box 2526 | Wellington 6140 | New Zealand
Web: www.mpi.govt.nz
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From: Official Information Act
Ministry for Primary Industries
Tēnā koe Stephan,
On behalf of Fleur Francois, Director, Diagnostics, Readiness and
Surveillance, please find attached a response to your Official Information
Act request.
Ngā mihi,
Official Information Act Team
Government Services | Public Affairs
Ministry for Primary Industries | Charles Fergusson Building, 34-38 Bowen
Street | PO Box 2526 | Wellington | New Zealand
Telephone: 0800 00 83 33 | Email: [1][email address] |
Web: [2]www.mpi.govt.nz
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2. http://www.mpi.govt.nz/
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Stephan Hokke left an annotation ()
I got a response and have to review it and perhaps pass it by a tech expert. It does not feel right, but it might be my lack of knowledge that lets me down in spotting the flaws which I feel are there.
Link to this